Filariasis IgG/IgM Rapid Test, Serum/Plasma, Filariasis parasites
Infectious Disease Test Kit
| Principle | Chromatographic Immunoassay |
| Format | Cassette |
| Specimen | WB/S/P |
| Certificate | CE |
| Reading Time | 15 minutes |
| Pack | 40 T |
| Storage Temperature | 2-30°C |
| Shelf Life | 2 Years |
A rapid test for the qualitative detection of IgG and IgM
antibodies to Filariasis parasites (W. Bancrofti and B. Malayi) in
human serum or plasma. For professional in vitro diagnostic use
only.
Applications:
The Filariasis IgG/IgM Rapid Test Cassette (Serum/Plasma) is a
rapid chromatographic immunoassay for the qualitative detection of
IgG and IgM antibodies to Filariasis parasites (W. Bancrofti and B.
Malayi) in serum or plasma to aid in the diagnosis of Filariasis
infection.
Description:
The lymphatic filariasis known as Elephantiasis, mainly caused by
W. bancrofti and B. malayi, affects about 120 million people over
80 countries . The disease is transmitted to humans by the bites of
infected mosquitoes within which the microflariae sucked from an
infected human subject develops into third-stage larvae. Generally,
repeated and prolonged exposure to infected larvae is required for
establishment of human infection.
The definitive parasitologic diagnosis is the demonstration of
microflariae in blood samples. However, this gold standard test is
restricted by the requirement for nocturnal blood collection and
lack of adequate sensitivity. Detection of circulating antigens is
commercially available. Its usefulness is limited for W. bancrofti
4 . In addition, microfilaremia and antigenemia develop from months
to years after exposure.
Antibody detection provides an early means to detect filarial
parasite infection. Presence of IgM to the parasite antigens
suggest current infection, whereas, IgG corresponds to late stage
of infection or past infection 5 . Furthermore, identification of
conserved antigens allows ‘pan- filaria’ test to be applicable.
Utilization of recombinant proteins eliminates cross-reaction with
individuals having other parasitic diseases 6 . The Filariasis
Rapid Test uses conserved recombinant antigens to simultaneously
detect antibody to the W. bancrofti and B. malayi parasites without
the restriction on specimen collection.
How to use?
Allow the test, specimen, buffer and/or controls to reach room
temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove
the test cassette from the sealed pouch and use it as soon as
possible. Best results will be obtained if the assay is performed
within one hour.
2. Place the cassette on a clean and level surface. Hold the
dropper vertically and transfer 1 drop of serum or plasma
(approximately 40 ul) to the specimen area, then add 2 drops of
buffer (approximately 80 ul ),and start the timer, see illustration
below.
3. Wait for the colored line(s) to appear. Read the result at 15
minutes, do not interpret the result after 20 minutes.
SUMMARY
The lymphatic filariasis known as Elephantiasis, mainly caused by
W. bancrofti and B. malayi, affects about 120 million people over
80 countries. The disease is transmitted to humans by the bites of
infected mosquitoes within which the microflariae sucked from an
infected human subject develops into third-stage larvae. Generally,
repeated and prolonged exposure to infected larvae is required for establishment of human infection.
The definitive parasitologic diagnosis is the demonstration of
microflariae in blood samples . However, this gold standard test is restricted by the requirement
for nocturnal blood collection and lack of adequate sensitivity. Detection of circulating antigens
is commercially available.
Its usefulness is limited for W. bancrofti. In addition, microfilaremia
and antigenemia develop from months to years after exposure. Antibody detection provides an early means to detect filarial
parasite infection. Presence of IgM to the parasite antigens suggest current infection, whereas,
IgG corresponds to late stage of infection or past infection5 . Furthermore, identification of
conserved antigens allows ‘pan filaria’ test to be applicable. Utilization of recombinant proteins
eliminates cross-reaction with individuals having other parasitic diseases .
The Filariasis Rapid Test uses conserved recombinant antigens to simultaneously detect antibody to the W.
bancrofti and B. malayi parasites without the restriction on specimen collection.
Order Information
| Cat. No. | Product | Specimen | Pack |
| IFIL-302 | Filariasis IgG/IgM Rapid Test Cassette | S / P | 40 T |
| IFIL-402 | Filariasis IgG/IgM Rapid Test Cassette | WB/S/P | 40 T |
