Carisoprodol Rapid Test Cassette (Urine), 2000ng/mL cut-off, GC/MS
method, DOA rapid test
| Principle | Chromatographic Immunoassay |
| Format | Dipstick, Cassette, Panel, Cup |
| Specimen | Urine |
| Certificate | CE |
| Reading Time | 5 minutes |
| Pack | 40T/50T |
| Storage Temperature | 2-30°C |
| Shelf Life | 2 Years |
| Sensitivity | 95.00% |
| Specificity | 94.20% |
| Accuracy | 94.40% |
| Cut-Off | 2000 ng/mL |
A rapid test for the qualitative detection of Carisoprodol in human
urine. For medical and other professional in vitro diagnostic use
only.
INTENDED USE
The Carisoprodol Rapid Test Cassette (Urine) is a rapid
chromatographic immunoassay for the detection of Carisoprodol in
human urine at a cut-off concentration of 2000ng/mL.
This assay provides only a qualitative, preliminary analytical test
result. A more specific alternate chemical method must be used in
order to obtain a confirmed analytical result. Gas
chromatography/mass spectrometry (GC/MS) is the preferred
confirmatory method. Clinical consideration and
professionaljudgment should be applied to any drug of abuse test
result, particularly when preliminary positive results are used.
SUMMARY
Carisoprodol, marketed under the brand name Soma among others, is a
medication used for musculoskeletal pain. Use is only approved for up to three
weeks. Effects generally begin within half an hour and last for up to six hours. It is taken by mouth.
Common side effects include headache, dizziness, and sleepiness.
Serious side effect may include addiction, allergic reactions, and seizures. In people with
a sulfa allergy certain formulations may result in problems. Safety during pregnancy and breastfeeding is
not clear. Meprobamate and other muscle-relaxing drugs often were subjects of misuse in the 1950s
and 60s.1,2 Overdose cases were reported as early as 1957, and have been reported on several occasions since
then.
Carisoprodol is metabolized by the liver and excreted by the
kidneys so this drug must be used with caution with patients that have impaired hepatic or renal function. Because
of potential for more severe side effects, this drug is on the list to avoid for elderly people.
The Carisoprodol Rapid Test is a rapid urine screening test that
can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively
detect elevated levels of Carisoprodol in urine. The Carisoprodol Rapid Test (Urine) yields a positive result
when Carisoprodol in urine exceeds 2000ng/mL.
PRINCIPLE
The Carisoprodol Rapid Test Cassette (Urine) is an immunoassay
based on the principle of competitive binding. Drugs which may be present in the urine specimen compete
against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary
action. Carisoprodol, if present in the urine specimen below 2000ng/mL, will not saturate the binding sites of
antibody-coated particles in the test. The antibody-coated particles will then be captured by immobilized
carisoprodol conjugate and a visible colored line will show up in the test line region. The colored line will
not form in the test line region if the Carisoprodol level exceeds 2000ng/mL because it will saturate all the binding
sites of anti-meperidine antibodies.
A drug-positive urine specimen will not generate a colored line in
the test line region because of drug competition, while a drug-negative urine specimen or a specimen
containing a drug concentration lower than the cut-off will generate a line in the test line region.
To serve as a procedural control, a colored line will always appear
in the control line region, indicating that proper volume of specimen has been added and membrane wicking has
occurred.
REAGENTS
Each test line contains anti-drug mouse monoclonal antibody and
corresponding drug-protein conjugates. The control line contains goat anti-rabbit IgG polyclonal antibodies
and rabbit IgG.
DIRECTIONS FOR USE
Allow the test, urine specimen, and/or controls to reach room
temperature (15-30ºC) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove
the test cassette from the sealed pouch and use it within one hour.
2. Place the test cassette on a clean and level surface. Hold the
dropper vertically and transfer 3 full drops of urine (approx. 120 uL) to the specimen well (S) of the
test cassette, and then start the timer. Avoid trapping air bubbles in the specimen well (S). See the
illustration below.
3. Wait for the color line(s) to appear. The result should be read
at 5 minutes. Do not interpret the result after 10 minutes.
INTERPRETATION OF RESULTS
(Please refer to the illustration)
NEGATIVE:* Two distinct colored lines appear. One colored line
should be in the control line region (C), and another colored line should be in the test line region (T).
This negative result indicates that the Carisoprodol concentration is below the detectable level
(2000ng/mL).
*NOTE: The shade of color in the test line region (T) will vary,
but it should be considered negative whenever there is even a faint colored line.
POSITIVE: One colored line appears in the control region (C). No
line appears in the test line region (T). This positive result indicates that the Carisoprodol concentration
exceeds the detectable level (2000ng/mL).
INVALID: Control line (C) fails to appear. Insufficient specimen
volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the
procedure and repeat the test using a new test. If the problem persists, discontinue using the lot immediately and
contact your local distributor.
EXPECTED VALUES
This negative result indicates that the Carisoprodol concentration
is below the detectable level of 2000ng/mL.Positive result means the concentration of Carisoprodol is above
the level of 2000ng/mL. The Carisoprodol Rapid Test Cassette has a sensitivity of 2000ng/mL.
Order Information
| Cat. No. | Product | Specimen | Pack |
| DCAR-102 | Carisoprodol (CAR) Rapid Test Cassette | Urine | 40 T |
| DCAR-114 | Carisoprodol(CAR) Rapid Test Panel | Urine | 40 T |
| DCAR-101 | Carisoprodol(CAR) Rapid Test Dipstick | Urine | 50 T |
