Calprotectin Rapid Test Cassette, Quantitative detection of Calprotectin in human fecal specimen

Quick Calprotectin Rapid Diagnostic Test For Profesional Use , Ce Approved

Application:

The Calprotectin Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Calprotectin in human feces specimen which might be useful for the diagnosis of inflammatory gastrointestinal disorders.

Description:

Calprotectin is a 24 kDa dimer of calcium binding proteins S100A8 and S100A9.The complex accounts for up to 60% of the soluble protein content of the neutrophil cytosol.Calprotectin becomes available in the intestinal lumen via leukocyte shedding,active secretion,cell disturbance, and cell death.

This results in elevated faecal calprotectin levels, which can be detected in the stool. Elevated faecal calprotectin levels therefore indicate migration of neutrophils into the intestinal mucosa, which occurs during intestinal inflammation.Faecal calprotectin has been used to detect intestinal inflammation, and can serve as a marker for inflammatory bowel diseases.Calprotectin is useful as a marker, as it is resistant to enzymatic degradation, and can be easily measured in faeces.

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

1. Exact QR Code from the QR code card for specific information of the test. Use only QR code card provided inside the test kits.

2. To collect fecal specimens:

Collect sufficient quantity of feces (1-2mL or 1-2g) in a clean, dry specimen collection container to obtain maximum antigens (if present). Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8℃ if not tested within 6 hours. For long term storage, specimens should be kept below -20℃.

3. To process fecal specimens:

• For Solid Specimens:

Unscrew the cap of the specimen collection tube,then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.

• For Liquid Specimens:

Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops (approximately 80μL) into the specimen collection tube containing the extraction buffer.

4. Tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Leave the tube alone for 2 minutes.

5. Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it within one hour. Best results will be obtained if the test is performed immediately after opening the foil pouch.

6. Hold the specimen collection tube upright and open the cap onto the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80μL) to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S). See illustration below.

7. Wait for the colored line(s) to appear. The result should be read with LF Reader at 5 minutes.

8. Do not interpret test results visually.

9. For LF Reader installation, start up and complete instructions refer to LF Reader User Manual. Operator must consult the LF Reader User Manual prior to use and become familiar with the processes and quality control procedures.

Note: If the specimen does not migrate (presence of particles), centrifuge the extracted specimens contained in the extraction buffer vial. Collect 80μL of supernatant, dispense into the specimen well (S) of a new test cassette and start afresh following the instructions mentioned above.

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