Allow the test, specimen, extraction buffer to equilibrate to room
temperature (15-30 °C) prior to testing.
1. Remove the test cassette from the sealed foil pouch and use it
as soon as possible. Best results will be obtained if the assay is
performed immediately after opening the foil pouch.
2. Collect nasal swabs from acute hyperthermia and untreated sick
pigs. The swab should be inserted into the nostril and rotated on
the inner wall 5-10 times to collect virus as much as possible.
Withdraw the sterile nasal swab from the nostril.
3. Remove the cover of the tube containing the extraction buffer.
See illustration 1. Place the tube in the workstation.Place the
swab specimen in the extraction tube. Rotate the swab for
approximately 10 seconds while pressing the head against the inside
of the tube to release the antigen in the swab. See illustration 2.
4. Remove the swab while squeezing the swab head against the inside
of the extraction tube as remove it to expel as much liquid as
possible from the swab. Discard the swab in accordance with local
biohazard waste disposal protocol. See illustration 3.
5. Tighten the extraction tube cap on top of the extraction tube.
Hold the specimen collection tube upright then unscrew the cap of
the specimen collection tube. See illustration 4.
6. Invert the specimen collection tube and add 3 drops of the
solution (approx.120 μL) to the sample well and then start the
timer.
7. Read the result at 15 minutes. Do not interpret the result after
20 minutes.
